Will ABI's Antibodies work in IFA?

All ABI antibodies work in IFA. Each is tested using infected cells of the appropriate virus that have been acetone fixed, PBS washed, and air dried on a teflon-masked microscope slide. The antibody is diluted to 10 µg per milliliter (1:100) in 1X IFA Wash Buffer, applied at 15 µl per well, and incubated at 37°C in a humid chamber for 30 minutes. The slide is gently rinsed in a stream of 1X IFA Wash Buffer and washed twice for five minutes each in a stirred 1X IFA Wash Buffer bath. Goat anti-mouse Ig-FITC is applied at 1 to 2 drops per well and incubated for 30 minutes as before. The slide is rinsed and washed as before, dried, and 1 to 4 drops IFA Mounting Solution applied per well before applying a #1 thickness cover slip. The slide is then observed at 400X using a fluorescent microscope with a halogen lamp and fluorescein filter set.