How pure are the elutriated monocyte suspensions?

Elutriated monocyte suspensions are >90% pure when analyzed by a variety of techniques. ABI documents purity during the elutriation process by multisizer analysis of purified monocytes, using statistical data to establish that >90% of the cell population has a mean cell size that is greater than small monocytes (approximately 8.5 microns). Final purity is confirmed by CD14 cell surface antigen distribution using immunofluorescence (IFA), with CD15 antigen tested by IFA as a control marker for granulocytes, which typically account for the majority of contaminating white blood cells in monocyte fractions. ABI customers with access to flow cytometry equipment have repeatedly confirmed ABI's monocyte purity estimates by this sensitive and reliable method.