10-601-001

Description

ABI Immunoassay Stabilizer is a proprietary mixture of non-toxic components that greatly enhances the long term stability of many immobilized antigens and antibodies. The solution is supplied ready to use and, in most cases, can replace your current blocking solution. When properly applied, immunoassay plates or membranes may be completely dried while sacrificing little, if any, of the activity of the fully hydrated protein.

Advantages

The increased antigen/antibody stability can be applied in several ways:

• Increased sensitivity of your assay.
• Lower cost per test due to decreased antigen use (antigen can be diluted further).
• Increased shelf life.
• Eliminate need for refrigeration of assay components.
• Reduced variability caused by improper shipping or storage.
• Numerous antigens and antibodies have been tested using Immunoassay Stabilizer. The results have varied, depending on the antigen or antibody used, from a doubling of the activity to a twenty-fold increase.

The stabilizer is sterile filtered (0.2 mm) and is supplied without preservatives. Use aseptic technique in dispensing the reagent, or add a preservative compatible with your assay. Economical volume pricing as well as custom formulations are available. Please call Advanced Biotechnologies Inc or your distributor for more information.

Recommended Protocol for Use

ABI Immunoassay Stabilizer can be readily incorporated into most immunoassay production protocols. Use the reagent as follows:

1. Immobilize or adsorb the antigen or antibody according to your established protocol and wash to remove excess and weakly bound material.
2. Immediately after washing, before the substrate has dried, add sufficient Immunoassay Stabilizer to contact all adsorbed protein. For example, if you used 100 μl per well of primary protein solution in step one, use 100 to 125 μl per well of Immunoassay Stabilizer.
3. Incubate at room temperature 30 minutes to allow interaction of the Immunoassay Stabilizer ingredients with the adsorbed protein.
4. Remove or aspirate the stabilizer. It is essential that a film of Immunoassay Stabilizer remains during the drying process, therefore, do not wash prior to drying.
5. Dry the assay components at room temperature for 1 hour to remove excess moisture. Membranes should be suspended to drip dry.
6. Dry the components for long-term storage. Recommended methods are:
• In a humidity controlled chamber or desiccator until dry (overnight).
• At 25°C for two hours in a vacuum oven.
7. Package in a vapor barrier pouch with a 2-gram desiccant pouch to ensure optimum stability of your immobilized antigen or antibody.

Quick Evaluation Protocol

To test Immunoassay Stabilizer, prepare two sets of plates or membranes using normal blocking buffer for one set and Immunoassay Stabilizer to block the second set. Dry and package the plate or membrane according to steps 5 to 7 of the above protocol. Incubate matched sets at 40°C and 50°C for three to five days. Test for activity by your assay procedure. Typically, there will be a dramatic increase in the activity of the stabilized surface compared to the unstabilized surface.

Annual purchase and large-volume contract pricing available upon request. Customized formulations available for special needs. Please inquire.

Delivery

Please allow one to two weeks for delivery. ELISA Stability of Selected Antigens and Antibodies in the Presence and Absence of Immunoassay Stabilizer.

Frequently Asked Questions

Please see the frequently asked questions here.