Name:
HTLV-I
MT-2 Infected Cell DNA
Virus:
HTLV-I, strain MT-2
Technical Data
PCR Analysis of DNA Control:
PCR analysis was performed on infected cell DNA using the Applied Biosystems Core Reagents PCR Kit and
primers specific to the rex and tax genes of HTLV-I. The reaction produced a 138 bp fragment. The PCR product
was visualized on a 1.0% agarose gel using EtBr staining. A representative gel photograph is show below.
Suspending Buffer:
10 mM Tris, 1 mM EDTA, pH 8.0
Storage Temperature:
-20°C
Shipping and Storage:
This product is shipped frozen on dry ice. Store at
-20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendation:
This DNA extraction procedure has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this lot was not specifically tested and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.
Human T-Cell Lymphotropic Virus Type I, strain MT-2 (HTLV-I). Family: Retroviridae, subfamily: Oncovirinae. Single-stranded, positive-sense enveloped RNA virus.
DNA isolated from HTLV-IMT-2 infected cells supplied at a concentration of 50 ng/µl cellular DNA in a volume of 100 µl. This DNA is not suitable for quantitation.
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