08-730-100

Name:

Adenovirus-2 Infected Cell DNA

Virus:

Human Adenovirus Type 2 (Ad-2)

Technical Data

Size:

100 µl

PCR Analysis of DNA Control:

PCR analysis was performed on infected cell DNA using the Applied Biosystems Core Reagents PCR Kit and primers specific for the hexon gene of Ad-2. The reaction produces a 205 bp fragment. The PCR product was visualized on a 1.0% agarose gel using EtBr staining.

Extract Preparation/DNA Quantitation:

SDS, proteinase K digestion followed by phenol/chloroform extraction and ethanol precipitation. DNA concentration was determined by fluorometry using Pico Green.

Storage Temperature:

-20°C

Shipping and Storage:

This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.

Safe Handling Recommendation:

This DNA extraction procedure has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this lot was not specifically tested and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.

Family: Adenoviridae. Nonenveloped, double-stranded DNA virus.

DNA isolated from Ad-2 infected cells supplied at a concentration of 50 ng/µl cellular DNA in a volume of 100 µl.

This DNA is not suitable for quantitation.

Documents

MSDS:

300-011.MSDS

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