Name:
CMV
AD169 Quantitated Viral DNA
Virus:
HCMV, strain AD169
Virus Preparation:
Sucrose density gradient purified virus
Technical Data
DNA Copy Number:
_________ DNA copies/µl (varies by lot, please
contact ABI for details)
CAUTION : ABI does not recommend storage of dilutions of these controls under any conditions. All dilutions should be made immediately before use and used promptly. We have observed that dilutions used for standard curves may tend to “lose” copy number with time (sometimes a matter of an hour or so after dilution), especially at dilutions less than 100-1000 copies per microliter.
Suspending Buffer:
10 mM Tris, 1 mM EDTA, pH 8.0
Storage Temperature:
-20°C
Shipping and Storage:
This product is shipped frozen on dry ice. Store at
-20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendation:
This DNA extraction procedure has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this lot was not specifically tested and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.
Human Cytomegalovirus, strain AD169 (HCMV, Human Herpesvirus 5, HHV-5). Family: Herpesviridae, subfamily: Betaherpesvirinae. Enveloped DNA virus.
Viral DNA isolated from sucrose gradient purified HCMVAD169 quantitated using real time PCR and supplied at a concentration of 1 to 2 × 104 copies/µl in 250 µl.
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