08-943-250

Name: 
JCVMAD1 Quantitated Viral DNA
Virus: 
JC Human Polyomavirus
Virus Preparation: 
Gel purified linear viral DNA from plasmid containing entire JCV genome. The JC viral genome is linearized at the BamH I site (base 4307) in the large T antigen gene. <strong>Note</strong>: Due to the linear nature of this DNA, primer sets that span the BamH I digestion site will not produce an amplification product.
Technical Data
Size: 
250 µl
DNA Copy Number: 
_________ DNA copies/µl (varies by lot, please contact ABI for details)
CAUTION: ABI does not recommend storage of dilutions of these controls under any conditions. All dilutions should be made immediately before use and used promptly. We have observed that dilutions used for standard curves may tend to “lose” copy number with time (sometimes a matter of an hour or so after dilution), especially at dilutions less than 100-1000 copies per microliter.
Suspending Buffer: 
10 mM Tris, 1 mM EDTA, pH 8.0
Extract Preparation/DNA Quantitation: 
Plasmid purified by alkaline lysis and ethanol precipitation followed by restriction enzyme digestion and purification on an agarose gel. Viral DNA copy number is determined by real time PCR using the Roche Light Cycler and primers specific for large T antigen of JC virus. DNA copy number may vary depending on the quantitation method used.
Storage Temperature: 
-20°C
Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendation: 
This DNA extraction procedure has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this lot was not specifically tested and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.

Gel purified linear viral DNA from a plasmid containing the entire JCV genome quantitated using real time PCR and supplied at a concentration of 1 to 2 × 105 copies/µl in 250 µl.

Documents
MSDS: 
300-011.MSDS
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