10-118-000

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Name: 
HIV-1IIIB Purified Virus
Biohazard Category: 
A
Storage Temperature: 
-70°C
Status: 
Current Product
Virus: 
HIV-1, strain IIIB
Virus Preparation: 
Sucrose density gradient purified virus
Cell Line for Propagation: 
H9
Technical Data
Size: 
1 mg
Suspending Buffer: 
10 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 7.5
Protein Concentration: 
[varies by lot] mg/ml by Pierce BCA protein assay method using BSA standard. Alternative methods for determining protein concentration may give different values.
Sterility: 
Negative for bacteria, yeast, fungi and Mycoplasma.
TCID_50 Titer: 
Varies by lot. contact ABI for details.
Virus Particle Count: 
Varies by lot. Contact ABI for details.
Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendations: 
This preparation contains ACTIVE VIRUS and should be handled in accordance with biosafety guidelines defined in NIH-CDC HHS publication No. (CDC) 93-8395.

Human Immunodeficiency Virus 1, strain IIIB (HIV-1). Family: Retroviridae, genus: Lentivirus. Single-stranded, positive-sense enveloped RNA virus.

Purified virus made from clarified, virus infected tissue culture harvest fluids that are concentrated and cleaned of debris by a combination of ultracentrifugation, ultrafiltration and selection of viral band from a sucrose density gradient. This preparation contains live virus.

IIIB is one of the original HIV-1 isolates; it is a group M lab-adapted virus and grows very well in transformed T-cell lines. It utilizes the CXCR4 receptor. HIV-1IIIB purified virus is generally not as infectious as HIV-1IIIB direct pelleted virus (Cat. No. 10-124-000).

Documents
MSDS: 
300-006.MSDS