Development of a LUX real-time PCR for the detection and quantification of human herpesvirus 7
| Title | Development of a LUX real-time PCR for the detection and quantification of human herpesvirus 7 |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Bergallo M, Costa C, Terlizzi M E, Sidoti F, Margio S, Astegiano S, Ponti R, Cavallo R | |
| Journal | Canadian Journal of Microbiology |
| Volume | 55 |
| No. | 3 |
| Pagination | 319-325 |
| Date Published | 03/2009 |
| Abstract | Human herpesvirus 7 is a highly seroprevalent beta-herpesvirus that, following primary infection, remains latent in CD4+ T cells and determines a persistent rather than a latent infection in various tissues and organs, including the lung and skin. This paper describes the development of an in-house light upon extension real-time PCR assay for quantification of human herpesvirus 7 DNA in clinical samples. The efficiency, sensitivity, specificity, inter- and intra-assay variability, and dynamic range have been determined. Subsequently, the assay has been validated by evaluating the human herpesvirus 7 load in bronchoalveolar lavages and skin specimens, chosen as 2 persistency sites, from healthy and pathological individuals. The real-time PCR assay developed in this study could be a useful tool to detect and quantify human herpesvirus 7 DNA in different clinical specimens to elucidate its epidemiological and pathogenic roles. |
| URL | http://rparticle.web-p.cisti.nrc.ca/rparticle/AbstractTemplateServlet?calyLang=eng&journal=cjm&volume=55&year=2009&issue=3&msno=w08-134 |
| DOI | 10.1139/W08-134 |
| Alternate Journal | Can. J. Microbiol. |
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