Category: Standard PCR Controls

HPV-16 Transformed Cell DNA

HPV-16 Transformed Cell DNA

Catalog #: 08-754-100

Size: 100 µL

Price: $615

To check product availability, please email info@abionline.com or call 800.426.0764 and reference the catalog #.

Description

Human Papillomavirus Type 16 transformed CaSki cell DNA PCR control.

For research use only. Not for use in diagnostic procedures.

Details

Shipping and Storage: This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.

Recommendations: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.

Safe Handling Recommendation: The DNA extraction procedure used has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested for infectivity and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.

FAQ

How are Standard PCR Controls supplied? Standard PCR Controls are supplied at a DNA concentration (viral plus cellular DNA) of 50 ng/µl. The concentration is determined by fluorometry.

Can a DNA copy number be determined for Standard PCR Controls? No, DNA in Standard PCR Controls is isolated from virus infected cells or virus transformed cells and therefore, these controls contain both cellular and viral DNA in unknown and variable proportions.

How is the DNA concentration of virus infected cell PCR Controls and virus transformed cell PCR Controls determined? The concentration of DNA is determined using fluorometry. Standard PCR Controls are intended primarily as positive controls in PCR reactions.

Why is the DNA concentration determined by fluorometry rather than the more common absorbance method? Fluorometric determinations virtually eliminate any skewing of the absorbance readings by interfering contaminants such as RNA, single-stranded DNA, oligonucleotides or nucleotides.

Documentation

Safety Data Sheet

Reference Articles

Davy C, Doorbar J. “Histological Analysis of Cervical Intraepithelial Neoplasia.“. In: Human Papillomaviruses. Vol. 119. New Jersey: Humana Press; 2006. Methods in Molecular Medicine™ 119.

Davy C, Doorbar J, Seth R, Rippin J, Guo L, Jenkins D. “Detection and Quantitation of HPV Gene Expression Using Real-Time PCR.“. In: Human Papillomaviruses. Vol. 119. New Jersey: Humana Press; 2005:. Methods in Molecular Medicine™ 119.

Yee C, Krishnan-Hewlett I, Baker CC, Schlegel R, Howley PM. “Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.” Am J Pathol. 1985;119:361-366.

Applications for use

  • PCR
  • Nucleic Acid-Based Assay

quality control testing includes

  • PCR
ABI QC Process