HIV-1 IIIB Strain Infected Cell DNA

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Catalog Number: 
08-707-000
Status: 
Current product--usually ships within 2 business days
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
100 µL
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-20˚C

Human Immunodeficiency Virus 1, strain IIIB (HIV-1). Family: Retroviridae, genus: Lentivirus. Single-stranded, positive-sense enveloped RNA virus. DNA isolated from HIV-1IIIB infected cells supplied at a concentration of 50 ng/µL cellular DNA in a volume of 100 µL. This DNA is not suitable for quantitation.

 

Virus: HIV-1, strain IIIB

Virus Preparation: Virus infected cells

Cell Line for Propagation: H9

Unit Size: 100 µL @ 50 ng/µL (5 µg) 100 µL @ 50 ng/µL (5 µg)

Suspending Buffer: 10 mM Tris, 1 mM EDTA, pH 8.0

DNA Quantitation: DNA concentration was determined by fluorometry using PicoGreen®.

PCR Analysis of DNA Control: PCR analysis was performed on infected cell DNA using the PE Biosystems Core Reagents PCR Kit and primers specific to the gag gene of HIV-1IIIB. The reaction produced a 114 bp fragment. The PCR product was visualized on a 1.0% agarose gel using EtBr staining. A representative photograph is shown below.

 

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendations: 
This DNA extraction procedure has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.
Documents
MSDS: 
300-011.MSDS