HTLV-II C3-44/Mo Strain Infected Cell DNA
Human T-Cell Lymphotropic Virus Type II, strain C3-44/Mo (HTLV-II). Family: Retroviridae, subfamily: Oncovirinae. Single-stranded, positive-sense enveloped RNA virus. DNA isolated from HTLV-IIC3-44/Mo infected cells supplied at a concentration of 50 ng/µL cellular DNA in a volume of 100 µL. This DNA is not suitable for quantitation.
Suspending Buffer: 10 mM Tris, 1mM EDTA, pH 8.0
DNA Quantitation: DNA concentration was determined by fluorometry using PicoGreen®.
PCR Analysis of DNA Quantitation: PCR analysis was performed on infected cell DNA using the PE Biosystems GeneAmp® Gold PCR Reagent Kit and primers specific to the pol gene of HTLV-II. The reaction produced a 104 bp fragment. The PCR product was visualized on a 1.0% agarose gel using EtBr staining.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.


