HPV-16 Transformed Cell DNA

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Catalog Number: 
08-754-100
Status: 
Current product--usually ships within 2 business days
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
100 µL
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-20˚C

Human Papilloma Virus Type 16 (HPV-16). Nonenveloped, double-stranded DNA virus. DNA isolated from HPV-16 transformed CaSki cells supplied at a concentration of 50 ng/µL cellular DNA in a volume of 100 µL. This DNA is not suitable for quantitation.

Virus: Human Papilloma Virus Type 16 

Cell Line for Propagation: CaSki--Note: CaSki cell DNA is reported to contain approximately 600 copies of integrated HPV-16 DNA per cell.

Suspending Buffer: 10 mM Tris, 1 mM EDTA, pH 8.0

DNA Quantitation: DNA concentration was determined by fluorometry using PicoGreen®.

PCR Analysis of DNA Control: PCR analysis was performed on infected cell DNA using the PE Biosystems GeneAmp® Gold PCR Reagent Kit and primers specific to the rex and tax genes of HTLV-I. The reaction produced a 138 bp fragment. The PCR product was visualized on a 1.0% agarose gel using EtBr staining.

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendations: 
This DNA extraction procedure has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.
Documents
MSDS: 
300-011.MSDS