HPV-18 Transformed Cell DNA

in
Catalog Number: 
08-755-100
Status: 
Call for availability
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
100 µL
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-20˚C

Human Papilloma Virus 18 (HPV-18). Nonenveloped, double-stranded DNA virus. DNA isolated from HPV-18 transformed HeLa cells supplied at a concentration of 50 ng/µL cellular DNA in a volume of 100 µL. This DNA is not suitable for quantitation.

Virus:  Human Papilloma Virus Type 18

Cell Line for Propagation:  HeLa--Note: HeLa cell DNA is reported to contain approximately 25-50 copies of integrated HPV-18 DNA per cell.

Suspending Buffer:  10 mM Tris, 1 mM EDTA, pH 8.0

PCR Analysis of DNA Control: PCR analysis was performed on purified HeLa DNA using the PE Biosystems GeneAmp® Gold PCR Reagent Kit and primers specific for HPV-18. The reaction produced a 140 bp fragment from the E6 region of HPV-18. The PCR product was visualized on a 1.0% agarose gel using EtBr staining.

DNA Quantitation:  DNA concentration was determined by fluorescence using PicoGreen®.

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendations: 
This DNA extraction procedure has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.
Documents
MSDS: 
300-011.MSDS