CMV AD169 Strain Quantitated Viral DNA

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CMVAD169 quantitated DNA PCR control.

Catalog Number: 
08-925-000
Status: 
Current product--usually ships within 2 business days
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
250 µL
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-20˚C

Virus: HCMV, strain AD169

Virus Preparation: Purified virus

Suspending Buffer: 10 mM Tris, 1 mM EDTA, pH 8.0

Cell Line for Propagation: Human foreskin fibroblasts (HFS)

PCR Analysis of DNA Control: PCR analysis was performed on purified CMVAD169 DNA using the PE Biosystems GeneAmp® Gold PCR Reagent Kit and primers specific to the glycoprotein 108 gene of CMV. The reaction produced a 292 bp fragment.

DNA Copy Number: 1-2 × 104 copies/µL (varies by lot, please contact ABI for details)

CAUTION: ABI does not recommend storage of dilutions of these controls under any conditions. All dilutions should be made immediately before use and used promptly. We have observed that dilutions used for standard curves may tend to "lose" copy number with time (sometimes a matter of an hour or so after dilution), especially at dilutions less than 100-1000 copies per microliter.

DNA Quantitation: Viral DNA copy number is determined by real time PCR1,2,3,4 using the Roche LightCycler®. DNA copy number may vary depending on the quantitation method used.

Application: ABI’s quantitated DNA controls are prepared from virus, bacteria, parasites, or mollicutes, and are intended for use as positive PCR quantitation standards for the organism in question.  Due to the nature of these products, ABI cannot guarantee their suitability as extraction controls.  Additionally, due to the extreme sensitivity of detection in PCR reaction, and since no method of purification can guarantee the complete absence of extraneous agents, DNA controls are not intended for use as negative controls for other organisms.

  1. 1. Stöcher M, Halwachs-Baumann G, Landt O, et al. "Parallel detection of five human herpes virus DNAs by a set of real-time polymerase chain reactions in a single run." Journal of Clinical Virology. 2003;26(1):85-93.
  2. 2. Jebbink J, Bai X, Rogers B B, Dawson BD, Scheuermann RH, Domiati-Saad R. "Development of Real-Time PCR Assays for the Quantitative Detection of Epstein-Barr Virus and Cytomegalovirus, Comparison of TaqMan Probes, and Molecular Beacons." J Mol Diagn. 2003;5:15-20.
  3. 3. Stocher M, Berg J. "Normalized Quantification of Human Cytomegalovirus DNA by Competitive Real-Time PCR on the LightCycler Instrument." Journal of Clinical Microbiology. 2002;40(12):4547-4553.
  4. 4. Ryan JL, Fan H, Glaser SL, Schichman SA, Raab-Traub N, Gulley ML. "Epstein-Barr Virus Quantitation by Real-Time PCR Targeting Multiple Gene Segments: A Novel Approach to Screen for the Virus in Paraffin-Embedded Tissue and Plasma." J Mol Diagn. 2004;6:378-385.
Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendations: 
This DNA extraction procedure has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.
Documents
MSDS: 
300-011.MSDS