VZV Rod Strain Quantitated Viral DNA
VZVRod quantitated DNA PCR control.
Virus: VZV, Rod strain
Virus Preparation: Direct pelleted virus
Cell Line for Propagation: Vero
Suspending Buffer: 10 mM Tris, 1 mM EDTA, pH 8.0
PCR Analysis of DNA Control: PCR analysis was performed on VZVRod DNA using the PE Biosystems GeneAmp® Gold PCR Reagent Kit and primers specific to the glycoprotein E gene of VZV. The reaction produced a 677 bp fragment.
DNA Copy Number: 1-2 × 104 copies/µL (varies by lot, please contact ABI for details)
DNA Quantitation: Viral DNA copy number is determined by real time PCR1 using the Roche LightCycler®. DNA copy number may vary depending on the quantitation method used.
Application: ABI’s quantitated DNA controls are prepared from virus, bacteria, parasites, or mollicutes, and are intended for use as positive PCR quantitation standards for the organism in question. Due to the nature of these products, ABI cannot guarantee their suitability as extraction controls. Additionally, due to the extreme sensitivity of detection in PCR reaction, and since no method of purification can guarantee the complete absence of extraneous agents, DNA controls are not intended for use as negative controls for other organisms.
- 1. "Parallel detection of five human herpes virus DNAs by a set of real-time polymerase chain reactions in a single run." Journal of Clinical Virology. 2003;26(1):85-93.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.



