Toxoplasma gondii RH Strain Quantitated DNA

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Catalog Number: 
08-948-250
Status: 
Current product--usually ships within 2 business days
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
250 µL
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-20˚C

Parasite: Toxoplasma gondii, strain RH

Tachyzoite Preparation: Tachyzoites concentrated from infected Vero cells

Suspending Buffer: 10 mM Tris, 1 mM EDTA, pH 8.0

DNA Copy Number: 1-2 × 104 copies/µL (varies by lot, please contact ABI for details)

CAUTION: ABI does not recommend storage of dilutions of these controls under any conditions. All dilutions should be made immediately before use and used promptly. We have observed that dilutions used for standard curves may tend to "lose" copy number with time (sometimes a matter of an hour or so after dilution), especially at dilutions less than 100-1000 copies per microliter.

PCR Analysis of DNA Control: PCR analysis was performed on purified Toxoplasma gondii DNA using the PE Biosystems GeneAmp® Gold PCR Reagent Kit and primers specific to the B1 gene of Toxoplasma gondii. The reaction produced a 619 bp fragment.

DNA Quantitation: DNA copy number is determined by real time PCR using the Roche LightCycler®. DNA copy number may vary depending on the quantitation method used.

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendations: 
This DNA extraction procedure has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.
Documents
MSDS: 
300-011.MSDS