HIV-1 IIIB Strain Purified Virus
UN 2814
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Defined by IATA in DG Regulations 3.6.2.2.2.1 as "An infectious substance which is transported in a form that, when exposure to it occurs, is capable of causing permanent disability, life-threatening or fatal disease in otherwise healthy humans or animals." This is not an indication of biosafety level, but of safety as relates to shipping in a properly prepared package.
Human Immunodeficiency Virus 1, strain IIIB (HIV-1). Family: Retroviridae, genus: Lentivirus. Single-stranded, positive-sense enveloped RNA virus.
Purified virus made from clarified, virus infected tissue culture harvest fluids that are concentrated and cleaned of debris by a combination of ultracentrifugation, ultrafiltration and selection of viral band from a sucrose density gradient. This preparation contains live virus.
IIIB is one of the original HIV-1 isolates; it is a group M lab-adapted virus and grows very well in transformed T-cell lines. It utilizes the CXCR4 receptor. HIV-1IIIB purified virus is generally not as infectious as HIV-1IIIB direct pelleted virus (Cat. No. 10-124-000).
Virus: HIV-1, strain IIIB
Virus Preparation: Purified virus
Cell Line for Propagation: H9
Suspending Buffer: 10 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 7.5
Final Fill Volume: Varies by lot
Virus Particle Count: Varies by lot
Protein Concentration: [Varies by lot] mg/mL by Pierce BCA protein assay method using BSA standard. Alternative methods for determining protein concentration may give different values.
Sterility: Bacteria, yeast, fungi and Mycoplasma were not detected by our assay methods.
TCID50 Titer: Varies by lot
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
