HIV-1 IIIB Strain Purified Viral Lysate

Human Immunodeficiency Virus 1, strain IIIB (HIV-1). Family: Retroviridae, genus: Lentivirus. Single-stranded, positive-sense enveloped RNA virus. HIV-1, strain IIIB purified virus preparation lysed with Triton X-100. Virus is completely inactivated and contains all viral proteins present in active preparation. Can be used for applications such as ELISA or Western blot requiring non-infectious viral proteins.

Virus: HIV-1, strain IIIB

Virus Preparation: Purified virus

Cell Line for Propagation: H9

Suspending Buffer: 10 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 7.5

Preparation of Lysate: HIV-1IIIB purified virus adjusted to a protein concentration of approximately 1.0 mg/mL and Triton X-100 added to a final concentration of 1% in TNE buffer.

Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.

Virus Particle Count: Varies by lot

Functional Activity: Reactive in Western blot at 5 µg using MAb HIV p24 (1:200) and at 40 µg using MAb HIV-1 gp120 (1:200). Reactive in ELISA.

Final Fill Volume: Varies by lot

Verification of Inactivation

Infectivity of Active Virus: [varies by lot] TCID₅₀/mL
Infectivity of Viral Lysate:  Zero

These lots of viral lysate are specifically tested for residual infectivity and no infectious virus are detected. This is demonstrated using a 28-day TCID₅₀ assay in H9 cells with endpoint determination by HIV-1 p24 antigen capture ELISA. The methods of inactivation used in preparation of this viral lysate have been validated to consistently produce a completely inactivated antigen preparation.