HIV-1 IIIB Strain Direct Pelleted Virus (100X)
UN 2814
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Defined by IATA in DG Regulations 3.6.2.2.2.1 as "An infectious substance which is transported in a form that, when exposure to it occurs, is capable of causing permanent disability, life-threatening or fatal disease in otherwise healthy humans or animals." This is not an indication of biosafety level, but of safety as relates to shipping in a properly prepared package.
Human Immunodeficiency Virus 1, strain IIIB (HIV-1). Family: Retroviridae, genus: Lentivirus. Single-stranded, positive-sense enveloped RNA virus. Direct pelleted virus concentrated 100X from clarified tissue culture supernatant without further purification. This preparation contains live virus. IIIB is one of the original HIV-1 isolates; it is a group M lab-adapted virus and grows very well in transformed T-cell lines. It utilizes the CXCR4 receptor. HIV-1IIIB direct pelleted virus is generally more infectious than HIV-1IIIB purified virus (Cat. No. 10-118-000).
Virus: HIV-1, strain IIIB
Cell Line for Propagation: H9
Concentration: 100×
Suspending Buffer: RPMI 1640 with 10% fetal bovine serum and 50 µg/mL gentamicin
Final Fill Volume: Varies by lot
Sterility: Bacteria, yeast, fungi and Mycoplasma were not detected by our assay methods.
TCID50 Titer: Varies by lot. HIV-1 is titered in H9 cells over a four-week period with endpoint determination by HIV-1 p24 antigen capture assay. Titer is calculated using Karber method.
