HIV-2 NIH-Z Strain Purified Virus
UN 2814
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Defined by IATA in DG Regulations 3.6.2.2.2.1 as "An infectious substance which is transported in a form that, when exposure to it occurs, is capable of causing permanent disability, life-threatening or fatal disease in otherwise healthy humans or animals." This is not an indication of biosafety level, but of safety as relates to shipping in a properly prepared package.
Sterility Mycoplasma TCID50 (106 or higher) Protein Concentration Virus Particle Count Human Immunodeficiency Virus 2, strain NIH-Z (HIV-2). Family: Retroviridae, genus: Lentivirus. Single-stranded, positive-sense enveloped RNA virus.
Lab adapted strain; grows well in transformed T-cell lines. HIV-2 causes disease similar to HIV-1 but is not as aggressive. Many people have been found infected with this virus without exhibiting overt disease. It is very similar to the related monkey virus SIV-2. Purified virus is a preparation made from clarified, virus infected tissue culture harvest fluids that are concentrated and cleaned of debris by a combination of ultracentrifugation, ultrafiltration and selection of viral band from a gradient. This preparation contains live virus.
Virus: HIV-2, strain NIH-Z
Virus Preparation: Purified virus
Cell Line for Propagation: HuT 78
Suspending Buffer: 10 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 7.5
Final Fill Volume: Varies by lot.
Virus Particle Count: Varies by lot.
Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.
Sterility: Tested negative for bacteria, yeast, fungi, and Mycoplasma
TCID50 Titer: Varies by lot
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
