CMV AD169 Strain Purified Viral Lysate (100 µg/mL)
Sterility Mycoplasma TCID50 ELISA Western blot Protein Concentration Cytomegalovirus, strain AD169 (CMV, Human Herpesvirus 5, HHV-5). Family: Herpesviridae, subfamily: Betaherpesvirinae. Enveloped DNA virus.
CMV, strain AD169 purified virus lysed with Triton X-100. Virus is completely inactivated and contains all viral proteins present in active preparation. Can be used for applications such as ELISA or Western blot requiring non-infectious viral proteins.
Virus: HCMV, strain AD169
Virus Preparation: Purified virus
Cell Line for Propagation: Human foreskin fibroblasts (HFS)
Suspending Buffer: 10 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 7.5
Preparation of Lysate: HCMV purified virus adjusted to a protein concentration of approximately 1.0 mg/mL and Triton X-100 added to a final concentration of 0.5% in TNE buffer.
Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.
Functional Activity: Reactive in Western blot using HCMV pp28 and gB MAbs. Reactive in ELISA with HCMV MAbs to pp65, pp28, and gB.
Final Fill Volume: Varies by lot
Validation of Inactivation: Varies by lot
This lot of viral lysate has been specifically tested for residual infectivity and no infectious virus was detected. The lysate was titered in HFS cells over a 12–14 day period with endpoint determination by cytopathic effect (CPE). This product has been treated using methods of inactivation that have been validated in vitro for complete inactivation of high-titered herpesviruses.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
