HSV-1 MacIntyre Strain Purified Viral Lysate
Herpes Simplex Virus Type 1, strain MacIntyre (HSV-1, Human Herpesvirus 1, HHV-1). Family: Herpesviridae, subfamily: Alphaherpesvirinae, genus: Simplexvirus. Enveloped DNA virus.
HSV-1, strain MacIntyre purified virus lysed with Triton X-100. Virus is completely inactivated and contains all viral proteins present in active preparation. Can be used for applications such as ELISA or Western blot requiring non-infectious viral proteins.
Virus: HSV-1, MacIntyre strain
Virus Preparation: Purified virus
Cell Line for Propagation: Vero
Suspending Buffer: 10 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 7.5
Preparation of Lysate: HSV-1 purified virus adjusted to a protein concentration of approximately 1.0 mg/mL and Triton X-100 added to a final concentration of 0.5 % in TNE buffer.
Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.
Virus Particle Count: Varies by lot.
Functional Activity: Reactive in Western blot. Reactive in ELISA using HSV-1 gG MAb.
Final Fill Volume: Varies by lot
Validation of Inactivation: These lots of viral lysate has been specifically tested for residual infectivity and no infectious virus was detected. The lysate was titered in Vero vells over a 7-day period with endpoint determination by cytopathic effects (CPE). This product has been treated using methods of inactivation that have been validated in vitro for complete inactivation of high-titered herpesviruses.
