RSV A-2 Strain Purified Virus (100 µg/mL)

in
Catalog Number: 
10-247-PNK
Status: 
Current product--usually ships within 2 business days
Size: 
1 mL
Biohazard Category: 
BBiological Substance, Category B
UN 3373
Shipment of this product requires that Advanced Biotechnologies has an Acceptance of Responsibility Form on file for the end user. Please click the "B" your mouse is hovering over for this form.

Defined by IATA in DG Regulations 3.6.2.2.2.2 as "An infectious substance which does not meet the criteria for inclusion in Category A." This is not an indication of biosafety level, but of safety as relates to shipping in a properly prepared package.
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-70˚C

Virus: RSV, strain A-2

Virus Preparation: Purified Virus

Cell Line for Propagation: HEp-2

Suspending Buffer: 10mM Tris, 150mM NaCl, 1mM EDTA, pH 7.5

Final Fill Volume: Varies by lot

Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.

TCID50 Titer: Varies by lot.
RSV is titered in HEp-2 cells over a period of 7 days with endpoint- determination by cytopathic effect (CPE).

Important Recommendation: Before use, rapidly thaw frozen vial in a water bath set at 37°C and then store on ice. Use pre-cooled medium/buffer for virus dilution and store on ice before inoculating into cell culture. For future use, aliquot into small amounts and freeze rapidly in a dry ice/alcohol bath and store at -70°C. Do NOT store virus stock at 2–8°C.

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Safe Handling Recommendations: 
This preparation contains ACTIVE VIRUS and should be handled in accordance with biosafety guidelines defined in NIH-CDC HHS publication No. (CDC) 93-8395.