Neisseria gonorrhoeae Viable Cells
UN 3373
Shipment of this product requires that Advanced Biotechnologies has an Acceptance of Responsibility Form on file for the end user. Please click the "B" your mouse is hovering over for this form.
Defined by IATA in DG Regulations 3.6.2.2.2.2 as "An infectious substance which does not meet the criteria for inclusion in Category A." This is not an indication of biosafety level, but of safety as relates to shipping in a properly prepared package.
Bacteria Preparation: Neisseria gonorrhoeae, concentrated bacteria from cultures. N. gonorrhoeae stock culture was inoculated onto Chocolate Agar media and incubated at 37° +/- 2°C under microaerophilic conditions. ID tests were performed after 48 hours culture growth.
Cell Density: Varies by lot
Suspending Buffer: Dulbecco's Phosphate Buffered Saline (DPBS), pH 7.2-7.4 + 10% Glycerol
ID Tests: Gram stain: Gram negative Diplococci; Oxidase test: Positive; Catalase test: Positive
Enumeration of Cell Numbers: 48 hours growth of N. gonorrhoeae on Chocolate Agar plates was suspended in DPBS and the cell numbers enumerated by spectrophotometry ( A550)2
DNA Extraction/Quantitation: Genomic DNA was prepared from bacterial cells using the QIAamp DNA Mini Kit. Bacterial DNA copy number is determined by real time PCR using the Roche LightCycler®. DNA copy number may vary depending on the quantitation method used.
Quantitative DNA PCR Analysis: [Varies by lot]copies/mL
