Neisseria gonorrhoeae Heat-Inactivated Cells

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Catalog Number: 
10-263-000
Status: 
Current product--usually ships within 2 business days
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
1 mL
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-70˚C

Bacterial Preparation: 48 hours growth of N. gonorrhoeae on Chocolate Agar plates was suspended in DPBS and the cell numbers enumerated by spectrophotometry (A550)1

Cell Density: Varies by lot

Suspending Buffer: Dulbecco’s Phosphate Buffered Saline (DPBS), pH 7.4 + 10% Glycerol

ID Tests: Gram stain: Gram negative Diplococci; Oxidase test: Positive; Catalase test: Positive

Enumeration of Cell Numbers: 48 hours growth of N. gonorrhoeae on Chocolate Agar plates was suspended in DPBS and the cell numbers enumerated by spectrophotometry (A550)2

Heat Inactivation: Cells of N. gonorrhoeae suspended in DPBS (2.75 × 108 cells/ml) were inactivated by heat at an optimum temperature and exposure time. 

DNA Extraction/Quantitation: Genomic DNA was prepared from bacterial cells using the QIAamp DNA Mini Kit. Bacterial DNA copy number is determined by real time PCR using the Roche LightCycler®. DNA copy number may vary depending on the quantitation method used.

Quantitative DNA PCR Analysis: [Varies by lot]copies/mL

  1. 1. Hanahan, D. J. Mol. Biol., 166 : 557–580, 1983.
  2. 2. Hanahan, D. J. Mol. Biol., 166 : 557–580, 1983.
Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Safe Handling Recommendations: 
This preparation of heat inactivated cells was prepared from viable bacteria. It was specifically tested for residual infectivity and validated to be negative for viable bacteria. This preparation should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.