Chlamydia trachomatis LGV-II 434 Strain Heat-Inactivated Elementary Bodies

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Catalog Number: 
10-265-000
Status: 
Current product--usually ships within 2 business days
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
1 mL
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-70˚C

Elementary Body Preparation: Purified elementary bodies (EB's)

EB Count by TEM: [varies by lot] EB/mL

Sterility: Bacteria, yeast, fungi and Mycoplasma were not detected by our assay methods.

Suspending Buffer: SPG buffer (10 mM Sodium phosphate, 220 mM Sucrose, 0.50 mM L-Glutamic acid) pH 6.8-7.0

Heat Inactivation: Purified elementary bodies of Chlamydia trachomatis (2.5 × 109 EB/mL) were inactivated by heat at an optimum temperature and exposure time.

Validation of Infectivity: Both active and heat-inactivated purified elementary bodies of Chlamydia trachomatis/LGV II (undiluted to 10-5 dilutions) were assayed for infectivity on Vero cell monolayers. The inoculated cells were examined microscopically for the evidence of specific cytopathic effect (CPE) and stained by FITC-conjugated genus- specific anti-Chlamydia monoclonal antibody on day 7 post- inoculation.

DNA Extraction/Quantitation: Genomic DNA was prepared from elementary bodies using the QIAamp DNA Mini Kit. DNA copy number is determined by real time PCR using the Roche LightCycler®.  Note: DNA copy number may vary depending on the quantitation method used.

Quantitative DNA PCR Analysis: [varies by lot] copies/mL

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Safe Handling Recommendations: 
This preparation of heat-inactivated EB's was specifically tested and found to be non-infectious in cell culture based assay. The preparation should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.