Human Influenza A/PR/8/34 Purified Virus (H1N1), β-PL Inactivated

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Catalog Number: 
10-267-500
Status: 
Current product--usually ships within 2 business days
Size: 
0.5 mg
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-70˚C

Human Influenza A/PR/8/34 (H1N1) is considered a prototype strain of H1N1 subtype of Influenza A viruses and is most commonly used for experimental infection of ferrets and mice to study viral pathogenesis, immune response, and testing antiviral compounds.

Virus: Influenza A/PR/8/34 (H1N1)

Virus Preparation: Purified Virus

Host for Propagation: Chicken embryos

Source Material: Influenza A infected allantoic fluid

Suspending Buffer: Dulbecco’s Phosphate Buffered Saline (DPBS), pH 7.2

Final Fill Volume: Varies by lot

Virus Particle Count: Varies by lot

Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.

Sterility: Bacteria, yeast, fungi and Mycoplasma were not detected by our assay methods.

Validation of Inactivation: Beta-Propiolactone (β-PL) is a DNA alkylating agent that has been shown to be effective in the inactivation of viruses. β-PL chemically modifies viral DNA without affecting the immunogenicity or protein structure. After treatment with B-PL, infectious influenza A/PR/8/34 was completely inactivated as validated by cell culture based assay but retained viral morphology (by TEM) and antigens (by hemagglutination assay).

HA Titer: Varies by lot

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Safe Handling Recommendations: 
This inactivated biological preparation should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.