Human Influenza A/PR/8/34 Purified Virus (H1N1), UV-Inactivated

Virus: Influenza A/PR/8/34 (H1N1)

Virus Preparation: Purified virus

Host for Propagation: Chicken emryos

Source Material: Influenza A infected allantoic fluid

Suspending Buffer: Dulbecco's Phosphate Buffered Saline (DPBS), pH 7.2

Final Fill Volume: Varies by lot

Virus Particle Count: Varies by lot

Protein Concentration: Varies by lot

Sterility: Bacteria, yeast, fungi and Mycoplasma were not detected by our assay methods.

Validation of Inactivation: After treatment with ultraviolet (UV) light irradiation, infectious Influenza A/PR/8/34 was completely inactivated as validated by cell culture (MDCK) based assay (active Influenza A/PR/8/34 had a TCID₅₀ titer of 10^9.75/ml), but retained viral morphology (by TEM) and hemagglutination antigen (by hemagglutination assay).

HA Titer: Varies by lot