CMV AD169 Strain Purified Virus, β-PL Inactivated

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Catalog Number: 
10-275-500
Status: 
New Product
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
0.5 mg
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-70˚C

Virus: HCMV, strain AD169

Virus Preparation: Purified virus

Cell Line for Propagation: Human foreskin fibroblasts (HFS)

Suspending Buffer: 10mM Tris, 150mM NaCl, 1mM EDTA, pH 7.5 with gentamicin (25 µg/mL)

Final Fill Volume: Varies by lot

Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.

Virus Particle Count: Varies by lot

Sterility: Bacteria, yeast, fungi and Mycoplasma were not detected by our assay methods.

Validation of Inactivation: Beta-Propiolactone (β-PL) is a DNA alkylating agent that has been shown to be effective in the inactivation of viruses.  B-PL chemically modifies viral DNA without affecting the immunogenicity or protein structure.  After treatment with β-PL, infectious HCMV, strain AD169 was completely inactivated as validated by cell culture based assay (active HCMV, strain AD169 had a TCID50 titer of 108.0 TCID50/mL), but retained viral morphology (by TEM).