Human Influenza A/2/Japan/305/57 (H2N2) Purified Virus, UV-Inactivated

Virus: Human Influenza A/2/Japan/305/57 (H2N2)

Virus Preparation: Purified virus

Host for Propagation: Chicken Embryos

Source Material: Influenza A infected allantoic fluid

Suspending Buffer: Dulbecco's Phosphate Buffered Saline (DPBS), pH 7.2

Final Fill Volume: Varies by lot

Virus Particle Count: Varies by lot

Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.

Sterility: Bacteria, yeast, fungi and Mycoplasma were not detected by our assay methods.

Validation of Inactivation: After treatment with ultraviolet (UV) light irradiation, infectious Influenza A/2/Japan/305/57 was completely inactivated as validated by cell culture (MDCK) based assay (active Influenza A/2/Japan/305/57 had a TCID₅₀ titer of 10^9.75/mL), but retained viral morphology (by TEM) and hemagglutination antigen (by hemagglutination assay).

HA Titer: Varies by lot