KSHV/HHV-8 MAb to ORF73 (anti-LNA-1)

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Catalog Number: 
13-210-100
Status: 
Current product--usually ships within 2 business days
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
100 µg
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-20˚C

Product Description: Rat monoclonal antibody to latent nuclear antigen (LNA-1) ORF-73 of human herpesvirus 8 (HHV-8). This antibody originates from ascites fluid and is purified by protein G chromatography.

Hybridoma Clone: LN 53

Fill Volume: Varies by lot.

Protein Concentration: Determined by BCA Assay using Bovine Gamma Globulin (BGG) as the standard. Alternative methods for determining protein concentration may give different results.

Presentation: Purified IgG supplied in PBS, pH 7.4, with 0.1% Thimerosal

Isotype: IgG2c

Purity: > 95% pure as estimated by analysis of Coomassie blue stained SDS-PAGE.

IFA Titer: Varies by lot.

Functional Activity: Reactive with latent protein ORF-73 of human herpesvirus 8 or HHV-8 positive cells. Reactive in IFA  with the nucleus of infected cells, ELISA, immunoprecipitation of specific viral protein, Western blot, and flow cytometry. Reactive in immunohistochemistry using paraffin-embedded sections from KS, PEL, and MCD patients.   Recommended use dilution: 1:1000 for IHC staining.

IHC Procedural Notes: For the demonstration of LNA-1 by IHC staining in formalin-fixed paraffin-embedded sections, perform antigen retrieval by heating in microwave oven in 10mM citrate buffer, pH 6.0 for 20 minutes.  Recommended buffer is CitraPlus Solution (BioGenex, San Ramon, CA, USA, www.biogenex.net).

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Documents
MSDS: 
300-013.MSDS