KSHV/HHV-8 MAb to ORF59

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Catalog Number: 
13-211-100
Status: 
Current product--usually ships within 2 business days
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
100 µg
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-20˚C

Product Description: Mouse monoclonal antibody to lytic cycle-associated early protein (50 kDa), encoded by ORF-59 of HHV-8, identified as DNA polymerase processivity factor (PF-8). This antibody originates from ascites fluid and is purified by protein G chromatography.

Hybridoma Clone: 11 D 1

Protein Concentration: Determined by BCA Assay using Bovine Gamma Globulin (BGG) as the standard. Alternative methods for determining protein concentration may give different results.

Presentation: Purified IgG supplied in PBS, pH 7.4 containing 0.1% sodium azide.

Isotype: IgG2b

Purity: > 95% pure as estimated by analysis of Coomassie blue stained SDS-PAGE.

IFA Titer: Varies by lot.

Functional Reactivity: This monoclonal antibody recognizes the HHV-8 lytic cycle- associated ORF-59 early protein (nuclear PF-8 protein), which is expressed in PEL cell lines (BCBL-1, BCP-1, BC-3, KS-1) 48 hours post-TPA induction. Reactive in IFA, Western blot (TPA-induced cell lysate), RIP, and in flow cytometry. Shows predominantly nuclear membrane fluorescence in IFA. No cross-reactivity with EBV.

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Documents
MSDS: 
300-013.MSDS