HHV-6 IgG Antibody IFA Kit

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Catalog Number: 
15-301-000
15-301-000 Representative Photograph
Status: 
Current product--usually ships within 2 business days
Size: 
Kit
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
2 to 8˚C

Intended Use

Advanced Biotechnologies Inc’s (ABI) indirect Immunofluorescent Assay (IFA) for Human Herpesvirus-6 (HHV-6) IgG antibody is intended for the qualitative and quantitative determination of IgG specific antibodies to HHV-6 in human serum or plasma. The test is intended as an aid in the detection of primary reactivation/reinfection or persistent infection with HHV-6 and can provide serological evidence of previous HHV-6 infection.

Principle of the Assay

ABI’s HHV-6 IgG IFA kit uses a rapid, simple, sensitive method for determination of IgG antibodies to Human Herpesvirus-6. ABI’s fluorescent antibody assay utilizes the indirect method of fluorescent antibody staining first described by Weller and Coons in 1954. The procedure is carried out in two basic reaction steps:

  1. Diluted human sera or plasma are added to the wells of IFA slides.  During incubation, diluted unknown samples and undiluted control samples are incubated with a mixture of HHV-6 infected and uninfected cells, which are attached to the slides.  Specific HHV-6 antibodies will bind to the antigen present in infected cells, formulating immunological complexes.  If the sample being examined contains no antibodies to HHV-6, no complex is formed.  After incubation, the IFA slides are washed to remove all unbound components. 
  2. Anti-human IgG conjugated to the dye Fluorescein Isothiocyanate (FITC) is added to each well, and during incubation, will bind to the antibody-antigen complexes present on the wells.  After incubation, each slide is washed to remove unbound conjugate.  After mounting, the slides are ready to evaluate under a fluorescent microscope.

If antibodies to HHV-6 are present (a positive reaction), bright apple-green fluorescence will be seen.

Kit Components

  1. HHV-6 10-Well slides (10 slides)
  2. Positive Control Plasma
  3. Negative Control Plasma
  4. FITC labeled anti-Human IgG
  5. 20× Wash Buffer
  6. Mounting Solution
  7. IFA Accessory Pack
  8. Package Insert (1 copy) or Download a Printable Kit Insert