HHV-7 IgG Antibody IFA Kit
Intended Use
Advanced Biotechnologies Inc’s (ABI) indirect Immunofluorescent Assay (IFA) for Human Herpesvirus-7 (HHV-7) IgG antibody is intended for the qualitative and quantitative determination of IgG specific antibodies to HHV-7 in human serum or plasma. The test is intended as an aid in the detection of primary reactivation/reinfection or persistent infection with HHV-7 and can provide serological evidence of previous HHV-7 infection.
Principle of the Assay
ABI’s HHV-7 IgG IFA kit provides a rapid, simple, sensitive assay utilizing the indirect method of fluorescent antibody staining first described by Weller and Coons in 1954. The procedure is carried out in two basic reaction steps:
- Diluted human sera or plasma are added to the wells of IFA slides. During incubation, diluted unknown samples and undiluted control samples are incubated with a mixture of HHV-7 infected and uninfected cells, which are attached to the slides. Specific HHV-7 antibodies will bind to the antigen present in infected cells, formulating immunological complexes. If the sample being examined contains no antibodies to HHV-7, no complex is formed. After incubation, the IFA slides are washed to remove all unbound components.
- Anti-human IgG conjugated to the dye Fluorescein Isothiocyanate (FITC) is added to each well, and during incubation, will bind to the antibody-antigen complexes present on the wells. After incubation, each slide is washed to remove unbound conjugate. After mounting, the slides are ready to evaluate under a fluorescent microscope.
If antibodies to HHV-7 are present (a positive reaction), bright apple-green fluorescence will be seen.
Kit Components
- HHV-7 10-Well slides (10 slides)
- Positive Control Plasma
- Negative Control Plasma
- FITC labeled anti-Human IgG
- 20× Wash Buffer
- Mounting Solution
- IFA Accessory Pack
- Package Insert (1 copy) or Download a Printable Kit Insert
