HHV-6 IgG Antibody ELISA Kit

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Catalog Number: 
15-401-000
Status: 
Current product--usually ships within 2 business days
Size: 
Kit
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
2 to 8˚C

Intended Use

Advanced Biotechnologies Inc’s (ABI) indirect Enzyme Linked ImmunoSorbent Assay (ELISA) for Human Herpesvirus 6 (HHV-6) IgG antibody is intended for the detection of HHV-6 IgG specific antibodies in human serum or plasma. The test is intended as an aid in the detection of reactivation/reinfection or persistent infection with HHV-6 and can provide serological evidence of previous HHV-6 infection.

Principle of the Assay

Advanced Biotechnologies Inc’s indirect HHV-6 IgG Antibody ELISA Kit is intended for the detection of HHV-6 IgG antibodies in human serum or plasma. 8-well strips of the ELISA plate are coated with HHV-6 antigen.  The test procedure involves three incubation steps:

  1. Tested human samples are added to the microtiter wells and any specific antibodies present to HHV-6 will bind to the antigen-coated plates, formulating immunological complexes. After the incubation, the strips are washed to remove unbound sample components.
  2. Anti-human IgG conjugated to the enzyme horseradish peroxidase (HRP) is added to the wells. During incubation, the HRP will bind to the antibody-antigen complexes present in the wells. After incubation, the wells are washed to remove unbound conjugate.
  3. HRP substrate tetramethylbenzidine (TMB) is added to the wells. During incubation, enzyme mediated cleavage of the substrate results in a color change. The reaction is stopped by adding Stop Solution. The color intensity of the solution is measured spectrophotometrically. The wells with positive samples (i.e., contain antibodies to HHV-6) will change color from blue to yellow. The wells with negative samples (i.e., do not contain antibodies to HHV-6) will remain almost colorless. The color intensity is proportional to the level of HHV-6 IgG antibodies in a sample.

Kit Contents

  • Antigen-Coated ELISA plate
  • Specimen Diluent
  • Positive Control Plasma
  • Negative Control Plasma
  • 20X Wash Buffer
  • HRP Conjugate Component A
  • HRP Conjugate Component B
  • Conjugate Substrate
  • Stop Solution
  • ELISA Accessory Pack
  • Package Insert (1 copy) or Download a Printable Kit Insert