KSHV/HHV-8 IgG Antibody ELISA Kit
Catalog Number:
15-501-000
Status:
Current product--usually ships within 2 business days
Size:
Kit
Use:
For research use only. Not for use in diagnostic procedures.
Storage Temperature:
2 to 8˚C Intended Use
Advanced Biotechnologies Inc’s Indirect Human Herpesvirus-8 (HHV-8) IgG antibody Enzyme Linked ImmunoSorbent Assay (ELISA) is intended for the detection of HHV-8 IgG specific antibodies in human serum or plasma.
Principle of the Assay
8-well strips are coated with optimal amounts of solubilized HHV-8 whole virus extract. The test procedure involves three incubation steps:
- Tested human samples are added to the microtiter wells and any specific antibodies present to HHV- 8 will bind to the antigen-coated plates, formulating immunological complexes. After the incubation, the strips are washed to remove unbound sample components.
- Anti-human IgG conjugated to the enzyme horseradish peroxidase (HRP) is added to the wells and during incubation, will bind to the antibody-antigen complexes already present in the wells. After incubation, the wells are washed to remove unbound conjugate.
- HRP substrate tetramethylbenzidine (TMB) is added to the wells. During incubation, enzyme mediated cleavage of the substrate results in a color change. The reaction is stopped by adding Stop Solution, and the wells with positive samples (i.e., contain antibodies to HHV-8) will change color from blue to yellow. The wells with negative samples (i.e., do not contain antibodies to HHV-8) will remain almost colorless. The color intensity is proportional to the level of HHV-8 IgG antibodies in a sample, and is measured spectrophotometrically.
Kit Contents
- Antigen-Coated ELISA plate
- 4X Specimen Diluent
- Positive Control Plasma
- Negative Control Plasma
- 20X Wash Buffer
- HRP Conjugate
- Conjugate Substrate
- Stop Solution
- ELISA Accessory Pack
- Package Insert (1 copy) or Download a Printable Kit Insert



